Biology (Grades 9–10) Midterm — Semester 1, Form B
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Free printable Biology midterm (Grades 9–10): 15 multiple-choice questions plus 1 written response covering Biochemistry, Cells & Cell Transport, Photosynthesis & Cellular Respiration. Answer key included — customize it in the Midterm Maker.
Biology (Grades 9–10) Midterm — Semester 1, Form B
Name: Date: Period:
Directions: Read each question carefully and choose the best answer. Answer the written response in complete sentences.
Covers: Biochemistry · Cells & Cell Transport · Photosynthesis & Cellular Respiration
Part 1: Multiple Choice
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How do enzymes speed up chemical reactions?
- A. By being used up as reactants
- B. By raising the temperature of the cell
- C. By lowering the activation energy
- D. By increasing the activation energy
-
What are the monomers of nucleic acids such as DNA and RNA?
- A. Monosaccharides
- B. Glycerol molecules
- C. Amino acids
- D. Nucleotides
-
Glucose belongs to which class of macromolecules?
- A. Proteins
- B. Lipids
- C. Nucleic acids
- D. Carbohydrates
-
The basic structure of a cell membrane is a bilayer of which molecules?
- A. Phospholipids
- B. Nucleotides
- C. Carbohydrates
- D. Proteins
-
When two monomers are joined by dehydration synthesis, which molecule is released?
- A. Oxygen
- B. Carbon dioxide
- C. Water
- D. Glucose
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Glucose moves into a cell through a carrier protein, down its gradient, without using ATP. What is this called?
- A. Exocytosis
- B. Active transport
- C. Facilitated diffusion
- D. Endocytosis
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What is a key difference between eukaryotic and prokaryotic cells?
- A. Eukaryotic cells lack ribosomes
- B. Prokaryotic cells lack DNA
- C. Prokaryotic cells have mitochondria
- D. Eukaryotic cells have a membrane-bound nucleus
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A plant cell placed in pure water swells but does not burst. Why?
- A. Water cannot enter plant cells
- B. Its cell wall resists the pressure
- C. It has no cell membrane
- D. Its chloroplasts absorb the water
-
A cube-shaped cell has sides of 2 µm. What is its surface area-to-volume ratio?
- A. 1:3
- B. 6:1
- C. 2:1
- D. 3:1
-
Diffusion is the movement of particles in which direction?
- A. From low concentration to high concentration
- B. Only into the nucleus
- C. From high concentration to low concentration
- D. Only when ATP is used
-
Where does glycolysis take place?
- A. Inner mitochondrial membrane
- B. Chloroplast
- C. Cytoplasm
- D. Mitochondrial matrix
-
The oxygen gas released during photosynthesis comes from which molecule?
- A. Water
- B. Chlorophyll
- C. Carbon dioxide
- D. Glucose
-
Which statement correctly relates photosynthesis and cellular respiration?
- A. The products of one are the reactants of the other
- B. Both processes release oxygen
- C. Plants carry out photosynthesis but not respiration
- D. Animals carry out both processes
-
Why do most plants appear green?
- A. Chlorophyll stores green light
- B. Chlorophyll produces green light
- C. Chlorophyll reflects green light
- D. Chlorophyll absorbs green light
-
What is the final electron acceptor in the electron transport chain?
- A. Water
- B. Glucose
- C. Oxygen
- D. Carbon dioxide
Part 2: Written Response
- A slice of potato is placed in very salty water and another in distilled water. Predict what happens to each and explain using osmosis and tonicity.
Answer Key — Biology (Grades 9–10) Midterm — Semester 1, Form B
- C — By lowering the activation energy Enzymes lower the energy barrier and are not consumed.
- D — Nucleotides Each nucleotide has a sugar, phosphate, and nitrogen base.
- D — Carbohydrates Glucose is a simple sugar, a carbohydrate monomer.
- A — Phospholipids Phospholipid heads face water; tails face inward.
- C — Water Dehydration synthesis removes a water molecule to form a bond.
- C — Facilitated diffusion Transport proteins help, but no cellular energy is spent.
- D — Eukaryotic cells have a membrane-bound nucleus Prokaryotes have DNA in a nucleoid region, not a nucleus.
- B — Its cell wall resists the pressure The rigid cell wall creates turgor pressure that prevents bursting.
- D — 3:1 Surface area 24 µm² ÷ volume 8 µm³ = 3:1.
- C — From high concentration to low concentration Particles spread out down their concentration gradient.
- C — Cytoplasm Glycolysis splits glucose in the cytoplasm.
- A — Water Water is split in the light-dependent reactions, releasing O₂.
- A — The products of one are the reactants of the other Plants and animals respire; plants also photosynthesize.
- C — Chlorophyll reflects green light Chlorophyll absorbs mainly red and blue light, reflecting green.
- C — Oxygen Oxygen accepts electrons and hydrogen ions to form water.
Written Response — Look-Fors
- Salty (hypertonic): water leaves cells, slice becomes limp/loses mass; distilled (hypotonic): water enters, slice becomes firm/gains mass; water moves from high to low water concentration; cell wall prevents bursting.